Toward continuous LC-MS analysis: surface modification of magnetic microparticles with TiO2 for phosphate adsorption

Issei Akutagawa; Yoshitake Akiyama; Yutaka Takahashi; Motoyuki Iijima; Yohei Okada; Hidehiro Kamiya; Kazuhiro Chiba

doi:10.1080/09168451.2014.905176

Toward continuous LC-MS analysis: surface modification of magnetic microparticles with TiO2 for phosphate adsorption

Biosci Biotechnol Biochem. 2014;78(5):748-54. doi: 10.1080/09168451.2014.905176. Epub 2014 May 15.

Authors

Issei Akutagawa¹, Yoshitake Akiyama, Yutaka Takahashi, Motoyuki Iijima, Yohei Okada, Hidehiro Kamiya, Kazuhiro Chiba

Affiliation

¹ a Department of Applied Biological Science , Tokyo University of Agriculture and Technology , Tokyo , Japan.

PMID: 25035973
DOI: 10.1080/09168451.2014.905176

Abstract

Continuous liquid chromatography-mass spectrometry (LC-MS) analysis was successfully demonstrated by using magnetic TiO2/Fe3O4 microparticles at the desalination interface. The particles could be prepared easily even on a practical scale at sufficient quality for efficient phosphate adsorption. Not only phosphate but several biomolecules were adsorbed onto the particles in a non-specific manner. Such samples could still be detected effectively in MS because the removal of phosphate derived from the LC eluent enhanced sample ionization and resulted in a significant reduction of phosphate cluster ions.

Keywords: continuous LC–MS analysis; desalination interface; magnetic TiO2/Fe3O4 microparticles; phosphate adsorption.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Chromatography, Liquid / methods*
Ferrosoferric Oxide / chemistry*
Mass Spectrometry / methods*
Microspheres*
Phosphates / chemistry*
Surface Properties
Titanium / chemistry*

Substances

Phosphates
titanium dioxide
Titanium
Ferrosoferric Oxide