The vaccine efficacy of recombinant duck enteritis virus expressing secreted E with or without PrM proteins of duck tembusu virus

Vaccine. 2014 Sep 15;32(41):5271-7. doi: 10.1016/j.vaccine.2014.07.082. Epub 2014 Aug 1.

Abstract

A newly emerged tembusu virus that causes egg-drop has been affecting ducks in China since 2010. Currently, no vaccine is available for this disease. A live attenuated duck enteritis virus (DEV; a herpesvirus) vaccine has been used routinely to control lethal DEV in ducks since the 1960s. Here, we constructed two recombinant DEVs by transfecting overlapping fosmid DNAs. One virus, rDEV-TE, expresses the truncated form of the envelope glycoprotein (TE) of duck tembusu virus (DTMUV), and the other virus, rDEV-PrM/TE, expresses both the TE and pre-membrane proteins (PrM). Animal study demonstrated that both recombinant viruses induced measurable anti-DTMUV neutralizing antibodies in ducks. After two doses of recombinant virus, rDEV-PrM/TE completely protected ducks from DTMUV challenge, whereas rDEV-TE only conferred partial protection. These results demonstrate that recombinant DEV expressing the TE and pre-membrane proteins is protective and can serve as a potential candidate vaccine to prevent DTMUV infection in ducks.

Keywords: Duck tembusu virus; Recombinant DEVs; Vector vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing / blood
  • Antibodies, Viral / blood
  • Coronavirus
  • Ducks / immunology
  • Flavivirus Infections / prevention & control
  • Flavivirus Infections / veterinary*
  • Flavivirus*
  • Neutralization Tests
  • Poultry Diseases / prevention & control*
  • Poultry Diseases / virology
  • Vaccines, Attenuated / immunology
  • Viral Envelope Proteins / immunology
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Vaccines, Attenuated
  • Viral Envelope Proteins
  • Viral Vaccines
  • prM protein, Flavivirus
  • glycoprotein E, Flavivirus