GLP-1 receptor agonist increases the expression of CTRP3, a novel adipokine, in 3T3-L1 adipocytes through PKA signal pathway

J Endocrinol Invest. 2015 Jan;38(1):73-9. doi: 10.1007/s40618-014-0156-8. Epub 2014 Aug 23.

Abstract

Objective: To investigate the effects of Exendin-4 (Ex-4), a glucagon-like peptide-1 (GLP-1) receptor agonist, on the expression of C1q/TNF-related protein-3 (CTRP3), a novel adipokine, in 3T3-L1 adipocytes. The role of protein kinase A (PKA) signal pathway in the effects was also investigated.

Methods: The mRNA and protein expressions of CTRP3 in 3T3-L1 adiocytes were detected by real-time polymerase chain reaction and western blot, respectively. Exendin-fragment 9-39 (Ex-9), a specific GLP-1 receptor antagonist, and H89, a selective antagonist of PKA, were used to confirm the signal pathway of Ex-4 on CTRP3.

Results: 2.5 or 5.0 nmol/l Ex-4 treatment for 8 h increased the expressions of CTRP3 mRNA and protein as well as PKA protein in 3T3-L1 adipocytes significantly, while Ex-9 or H89 blocked the up-regulation of CTRP3 expression induced by Ex-4 completely.

Conclusion: GLP-1 receptor agonist increases the expression of CTRP3 mRNA and protein in 3T3-L1 adipocytes via PKA signal pathway.

Keywords: C1q/TNF-related protein-3 (CTRP3); Glucagon-like peptide-1 (GLP-1); Protein kinase A (PKA).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / drug effects
  • Adipocytes / metabolism*
  • Adipokines / biosynthesis*
  • Adipokines / genetics
  • Animals
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • Exenatide
  • Gene Expression Regulation
  • Glucagon-Like Peptide 1 / agonists*
  • Glucagon-Like Peptide 1 / metabolism
  • Mice
  • Peptides / pharmacology*
  • Signal Transduction / drug effects
  • Signal Transduction / physiology*
  • Venoms / pharmacology*

Substances

  • Adipokines
  • CORS26 protein, mouse
  • Peptides
  • Venoms
  • Glucagon-Like Peptide 1
  • Exenatide
  • Cyclic AMP-Dependent Protein Kinases