Histogenesis of keratoacanthoma: histochemical and immunohistochemical study

Vivian Petersen Wagner; Manoela Domingues Martins; Caroline Siviero Dillenburg; Luise Meurer; Rogerio Moraes Castilho; Cristiane Helena Squarize

doi:10.1016/j.oooo.2014.10.006

Histogenesis of keratoacanthoma: histochemical and immunohistochemical study

Oral Surg Oral Med Oral Pathol Oral Radiol. 2015 Mar;119(3):310-7. doi: 10.1016/j.oooo.2014.10.006. Epub 2014 Oct 18.

Authors

Vivian Petersen Wagner¹, Manoela Domingues Martins¹, Caroline Siviero Dillenburg¹, Luise Meurer², Rogerio Moraes Castilho³, Cristiane Helena Squarize⁴

Affiliations

¹ Department of Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil 90035.
² Department of Pathology, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil 90035.
³ Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA 48109.
⁴ Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan, USA 48109. Electronic address: Csquariz@umich.edu.

PMID: 25488010
DOI: 10.1016/j.oooo.2014.10.006

Abstract

Objectives: Keratoacanthoma (KA), a keratinocytic neoplasm, is associated with sun exposure and is often found in the head and neck area, including the lip. KA is thought to arise from hair follicle cells, but its origin is largely unknown. Keratins (Ks) and histochemical stains are of great value to characterize and identify normal and neoplastic cells. The objective of this study is to analyze a panel of Ks and periodic acid-Schiff (PAS) staining on KA.

Study design: Using KA biopsies from the lips and normal skin samples, we performed immunohistochemical and histochemical profiling to determine which biomarkers are conserved between tumors and normal tissues.

Results: The normal hair follicle has multiple well-defined compartments. The outer root sheath (ORS) cells presented K6 and K14 and were also PAS positive. In addition, the infundibulum cells showed positive labeling to K10. Hair cortex keratin was observed in the cortical and precortical cells. Interestingly, KA tumor cells were positive for PAS, K6, K10, and K14 but not to hair cortex keratin. Lip and skin epithelium were negative for PAS and K6.

Conclusions: Our results indicate that KA of the lip is derived from ORS cells, particularly those cells associated with the upper ORS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Biomarkers, Tumor / analysis
Biopsy
Female
Hair Diseases / pathology*
Humans
Immunohistochemistry
Keratoacanthoma / pathology*
Male
Middle Aged
Periodic Acid-Schiff Reaction

Substances

Biomarkers, Tumor