Abstract
We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the violacein and carotenoid biosynthesis pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biosynthetic Pathways / genetics
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Carotenoids / metabolism
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Enzymes / genetics
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Enzymes / metabolism*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal
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Indoles / metabolism
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Multigene Family / genetics
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Pichia / enzymology*
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Pichia / genetics
Substances
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Enzymes
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Fungal Proteins
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Indoles
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Carotenoids
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violacein