Isolation and characterization of a novel invertebrate iridovirus from adult Anopheles minimus (AMIV) in China

J Invertebr Pathol. 2015 May:127:1-5. doi: 10.1016/j.jip.2015.01.007. Epub 2015 Jan 28.

Abstract

An invertebrate iridovirus (designated AMIV) was isolated from adult wild-captured Anopheles minimus mosquitoes in China. AMIV was pathologically and morphologically characterized and sequenced using the Ion Torrent™ sequencing platform. Phylogenetic analysis based on both the major capsid protein and core genes revealed that AMIV differs from all the members of the family Iridoviridae. The AMIV negatively strained virion has a diameter of about 130nm. AMIV contains a linear DNA molecule of 163,023bp, with 39% G+C content and 148 coding sequences. The genome analysis revealed that AMIV genome encodes a high content of replication associated genes including BRO-like genes. This is the ninth complete genome of IIV reported.

Keywords: Complete genome; Cytopathic effect; Electron microscope; Invertebrate iridovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anopheles / virology*
  • Base Sequence
  • China
  • DNA, Viral
  • Genome, Viral
  • Iridovirus / physiology*
  • Phylogeny
  • Sequence Analysis, DNA

Substances

  • DNA, Viral