Precise characterization of GlnR Box in actinomycetes

Biochem Biophys Res Commun. 2015 Mar 13;458(3):605-607. doi: 10.1016/j.bbrc.2015.02.010. Epub 2015 Feb 13.

Abstract

GlnR has been characterized as a central regulator governing most nitrogen metabolisms in many important actinomycetes. So far, the GlnR binding consensus sequences have been extensively studied, but with different motifs proposed, which has therefore brought confusion and impeded the understanding of the in-depth molecular mechanisms of GlnR-mediated transcriptional regulation. Here, a 30-nt GlnR-protected DNA sequence in the promoter of glnA in Amycolatopsis mediterranei was employed for precise characterization of GlnR binding consensus sequences. Site-by-site mutagenesis strategy combining with the Electrophoretic Mobility Shift Assay were employed, and a 5-nt GlnR Box was precisely defined as the basic unit for GlnR binding.

Keywords: Actinomycetes; GlnR; GlnR Box.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / genetics*
  • Actinobacteria / metabolism
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Consensus Sequence
  • DNA, Bacterial / genetics*
  • DNA, Bacterial / metabolism
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Glutamate-Ammonia Ligase / genetics*
  • Glutamate-Ammonia Ligase / metabolism
  • Nitrogen / metabolism
  • Promoter Regions, Genetic*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • glutamine synthetase I
  • Glutamate-Ammonia Ligase
  • Nitrogen