Abstract
p60 is a cellular protein that binds to the adenovirus E1A protein complex in virally infected or transformed human cells. In both infected and uninfected cells, p60 was found in a complex with the cdc2 protein kinase. Immune complexes containing p60 and cdc2 display a cell cycle-dependent histone H1 kinase activity that is most active in interphase. The previously described cdc2-p62/cyclin complex also acts as a histone H1 kinase but is maximally active in mitotic metaphase. The shift in the timing of activation of different cdc2-containing complexes suggests that each might play a distinct role in regulation of the cell cycle.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenovirus Early Proteins
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CDC2 Protein Kinase
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Cell Cycle
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DNA-Binding Proteins / metabolism*
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Electrophoresis, Gel, Two-Dimensional
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HeLa Cells
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Humans
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Macromolecular Substances
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Molecular Weight
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Nuclear Proteins / metabolism*
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Oncogene Proteins, Viral / metabolism*
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Phosphoproteins / metabolism*
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Precipitin Tests
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Proliferating Cell Nuclear Antigen
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Protamine Kinase / metabolism
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Protein Binding
Substances
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Adenovirus Early Proteins
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DNA-Binding Proteins
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Macromolecular Substances
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Nuclear Proteins
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Oncogene Proteins, Viral
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Phosphoproteins
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Proliferating Cell Nuclear Antigen
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Protamine Kinase
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CDC2 Protein Kinase