The effects and mechanisms of clinorotation on proliferation and differentiation in bone marrow mesenchymal stem cells

Biochem Biophys Res Commun. 2015 May 1;460(2):327-32. doi: 10.1016/j.bbrc.2015.03.034. Epub 2015 Mar 21.

Abstract

Data from human and rodent studies have demonstrated that microgravity induces observed bone loss in real spaceflight or simulated experiments. The decrease of bone formation and block of maturation may play important roles in bone loss induced by microgravity. The aim of this study was to investigate the changes of proliferation and differentiation in bone marrow mesenchymal stem cells (BMSCs) induced by simulated microgravity and the mechanisms underlying it. We report here that clinorotation, a simulated model of microgravity, decreased proliferation and differentiation in BMSCs after exposure to 48 h simulated microgravity. The inhibited proliferation are related with blocking the cell cycle in G2/M and enhancing the apoptosis. While alterations of the osteoblast differentiation due to the decreased SATB2 expression induced by simulated microgravity in BMSCs.

Keywords: BMSCs; Differentiation; Proliferation; SATB2; Simulated microgravity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Bone Marrow Cells / cytology*
  • Cell Cycle
  • Cell Differentiation*
  • Cell Line
  • Cell Proliferation*
  • DNA Primers
  • Mesenchymal Stem Cells / cytology*
  • Mice
  • Mice, Inbred C3H
  • Reverse Transcriptase Polymerase Chain Reaction
  • Weightlessness

Substances

  • DNA Primers