Allele frequencies of 23 autosomal short tandem repeat loci in the Philippine population

Jae Joseph Russell Beltran Rodriguez; Jazelyn M Salvador; Gayvelline C Calacal; Rita P Laude; Maria Corazon A De Ungria

doi:10.1016/j.legalmed.2015.02.005

Allele frequencies of 23 autosomal short tandem repeat loci in the Philippine population

Leg Med (Tokyo). 2015 Jul;17(4):295-7. doi: 10.1016/j.legalmed.2015.02.005. Epub 2015 Mar 14.

Authors

Jae Joseph Russell Beltran Rodriguez¹, Jazelyn M Salvador², Gayvelline C Calacal², Rita P Laude³, Maria Corazon A De Ungria⁴

Affiliations

¹ DNA Analysis Laboratory, Natural Sciences Research Institute, College of Science, University of the Philippines, Diliman, Quezon City, Philippines; Genetics and Molecular Biology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines, Los Baños, Laguna, Philippines.
² DNA Analysis Laboratory, Natural Sciences Research Institute, College of Science, University of the Philippines, Diliman, Quezon City, Philippines.
³ Genetics and Molecular Biology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines, Los Baños, Laguna, Philippines.
⁴ DNA Analysis Laboratory, Natural Sciences Research Institute, College of Science, University of the Philippines, Diliman, Quezon City, Philippines. Electronic address: mariadeungria@gmail.com.

PMID: 25804725
DOI: 10.1016/j.legalmed.2015.02.005

Abstract

We characterized diversity and forensic descriptive parameters of 23 autosomal STR loci (CSF1PO, D13S317, D16S539, D5S818, D7S820, TPOX, D18S51, D21S11, D3S1358, D8S1179, FGA, TH01, vWA, D1S1656, D10S1248, D12S391, D2S441, D22S1045, D19S433, D2S1338, D6S1043, Penta D and Penta E) among 167 unrelated Filipinos. The most variable autosomal STR loci observed is Penta E (observed heterozygosity: 0.9222, match probability: 0.0167). Results reveal matching probability of 8.21×10(-28) for 23 autosomal STR loci. This dataset for the Philippine population may now be used in evaluating the weight of DNA evidence for forensic applications such as in human identification, parentage/kinship testing, and interpretation of DNA mixtures.

Keywords: Allele frequency; Philippines; Population database; PowerPlex® 21 system; Short tandem repeat; Verifiler™ direct PCR amplification kit.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Asian People / ethnology
Asian People / genetics
DNA Fingerprinting / methods
Forensic Genetics / methods
Gene Frequency*
Genetic Loci
Genetic Variation*
Genetics, Population*
Humans
Male
Microsatellite Repeats / genetics*
Philippines
Polymerase Chain Reaction