Comparison of saliva PCR assay versus rapid culture for detection of congenital cytomegalovirus infection

Pediatr Infect Dis J. 2015 May;34(5):536-7. doi: 10.1097/INF.0000000000000609.

Abstract

As part of the CMV and Hearing Multicenter Screening (CHIMES) study, 72,239 newborns were screened for cytomegalovirus by rapid culture and real-time PCR of saliva samples. Of the 266 infants with congenital cytomegalovirus infection, discordance between rapid culture and PCR was observed in 14 children, and 13 were identified only by PCR, demonstrating the superiority of the PCR assay.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Cytomegalovirus Infections* / congenital
  • Cytomegalovirus Infections* / diagnosis
  • Cytomegalovirus Infections* / virology
  • Humans
  • Infant, Newborn
  • Prospective Studies
  • Real-Time Polymerase Chain Reaction / methods*
  • Saliva / virology*
  • United States
  • Viral Load
  • Virology / methods*