Serological evidence of influenza A viruses in frugivorous bats from Africa

PLoS One. 2015 May 12;10(5):e0127035. doi: 10.1371/journal.pone.0127035. eCollection 2015.

Abstract

Bats are likely natural hosts for a range of zoonotic viruses such as Marburg, Ebola, Rabies, as well as for various Corona- and Paramyxoviruses. In 2009/10, researchers discovered RNA of two novel influenza virus subtypes--H17N10 and H18N11--in Central and South American fruit bats. The identification of bats as possible additional reservoir for influenza A viruses raises questions about the role of this mammalian taxon in influenza A virus ecology and possible public health relevance. As molecular testing can be limited by a short time window in which the virus is present, serological testing provides information about past infections and virus spread in populations after the virus has been cleared. This study aimed at screening available sera from 100 free-ranging, frugivorous bats (Eidolon helvum) sampled in 2009/10 in Ghana, for the presence of antibodies against the complete panel of influenza A haemagglutinin (HA) types ranging from H1 to H18 by means of a protein microarray platform. This technique enables simultaneous serological testing against multiple recombinant HA-types in 5 μl of serum. Preliminary results indicate serological evidence against avian influenza subtype H9 in about 30% of the animals screened, with low-level cross-reactivity to phylogenetically closely related subtypes H8 and H12. To our knowledge, this is the first report of serological evidence of influenza A viruses other than H17 and H18 in bats. As avian influenza subtype H9 is associated with human infections, the implications of our findings from a public health context remain to be investigated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Chiroptera / blood
  • Chiroptera / immunology*
  • Chiroptera / virology
  • Disease Reservoirs / virology
  • Female
  • Ghana
  • Hemagglutinin Glycoproteins, Influenza Virus / immunology*
  • Influenza A virus / classification*
  • Influenza A virus / immunology
  • Male
  • Orthomyxoviridae Infections / immunology
  • Orthomyxoviridae Infections / veterinary
  • Orthomyxoviridae Infections / virology
  • Phylogeny

Substances

  • Antibodies, Viral
  • Hemagglutinin Glycoproteins, Influenza Virus

Grants and funding

This project was funded by the European Commission's FP7 program under the umbrella of the Antigone project - ANTIcipating the Global onset of Novel Epidemics (project number 278976, http://antigonefp7.eu/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.