Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses

J Clin Microbiol. 2015 Aug;53(8):2722-6. doi: 10.1128/JCM.01224-15. Epub 2015 May 27.

Abstract

Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Child
  • Child, Preschool
  • Coronavirus / classification*
  • Coronavirus / genetics
  • Coronavirus / isolation & purification*
  • Coronavirus Infections / diagnosis*
  • Coronavirus Infections / virology
  • Female
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Oligonucleotide Probes / genetics*
  • Oligonucleotides / genetics*
  • Real-Time Polymerase Chain Reaction / methods*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Young Adult

Substances

  • Oligonucleotide Probes
  • Oligonucleotides
  • locked nucleic acid