Malarial parasite detoxifies the heme generated in its food vacuole in many ways one of which involves heme polymerization to hemozoin. The existing heme polymerization assays involve use of activators along with buffers for polymerization of heme leading to its precipitation. Such assays then involve special instruments and laborious work of isolating the precipitated polymer and its detection. Simple and precise spectrophotometric and HTS methods were developed for heme polymerization using tween 20 as the activator without isolation of polymerized heme.
Keywords: Chloroquine; Heme; Hemozoin; Malaria; Tween 20; β-Hematin.
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