Anti-tumor effect of LTA combined with 5-FU on H22 tumor bearing mice

Bin Wang; Chang-Jiang Lei; Rong Wu; Lei Li; Chun-Mei Deng; Wen-Xia Chen; Fu-Rong Hu; Hao-Cheng Long; Ze-Zhang Tao; Cheng Zeng; Jian-Bin Huang; Chun-Zhou Chen; De-Fa Ren

doi:10.1016/j.apjtm.2015.07.002

Anti-tumor effect of LTA combined with 5-FU on H22 tumor bearing mice

Asian Pac J Trop Med. 2015 Jul;8(7):560-4. doi: 10.1016/j.apjtm.2015.07.002. Epub 2015 Jul 9.

Authors

Affiliations

¹ Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
² Department of General Surgery, The Second Affiliated Hospital of Jianghan University, Wuhan 430050, Hubei Province, China.
³ Department of Hepatobiliary Surgery, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China.
⁴ Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. Electronic address: taozezhang@hotmail.com.

PMID: 26276288
DOI: 10.1016/j.apjtm.2015.07.002

Abstract

Objective: To study the effect of lipoteichoic acid (LTA) and 5-FU on the expression of caspase-3, EGFR, TGF-α proteins of tumor tissue of H22 cancer bearing mice and its anti-tumor mechanism.

Methods: A total of 40 SPF grade Kunming mice were selected to establish H22 liver cancer model, and then the mice were divided into 4 groups at random with ten mice in each group. Group A was given saline lavage treatment, Group B was treated with 5-FU by intraperitoneal injection, Group C was treated with LTA by lump body injection; Group D was treated with LTA by lump body injection and 5-FU by intraperitoneal injection. Two weeks after the treatment, the mice in each group were executed and the tumor tissue was stripping and weighted, and the tumor growth inhibition ratio was calculated. Then the tumor tissue was processed for conventional embedding, sectioned to observe the expression of caspase-3, EGFR, TGF-α by immunohistochemical staining method.

Results: The tumor inhibitory rate o f Group D was significantly higher than Groups B and C (P < 0.05); B, the tumor inhibitory rate o f Group B had no statistical difference compared with Group C (P > 0.05). The IDO values of TGF-α, EGFR proteins in Groups B, C, D mice tumor tissue were significantly lower than that in group A (P < 0.05); while IDO value of caspase-3 in Groups B, C, D group mice tumor tissue was significantly higher than that in Group A (P < 0.05). The IDO value of TGF-α, EGFR in Group D mice tumor tissue were significantly lower than that in Groups B and C; While IDO value of aspase-3 in Group D was significantly higher than that in Groups B and C (P < 0.05).

Conclusions: LTA combined with 5-FU can effectively inhibit the tumorigenesis of H22 tumor bearing mice, increase the caspase-3 protein expression, inhibit TGF-α and EGFR protein expression, further promote tumor cell apoptosis and play a synergistic antitumor effect.

Keywords: 5-FU; Caspase-3; EGFR; LTA; TGF-α; Tumor inhibition rate.