Enzyme transition states from theory and experiment

Biochim Biophys Acta. 2015 Nov;1854(11):1727-8. doi: 10.1016/j.bbapap.2015.08.006. Epub 2015 Aug 22.

Authors

Mike E Harris¹, Joseph A Piccirilli², Darrin M York³

Affiliations

¹ Integration of kinetic isotope effect analyses to elucidate ribonuclease mechanism.
² Heavy atom labeled nucleotides for measurement of kinetic isotope effects.
³ Effect of Zn(2+) binding and enzyme active site on the transition state for RNA 2'-O-transphosphorylation interpreted through kinetic isotope effects.

PMID: 26302659
DOI: 10.1016/j.bbapap.2015.08.006

No abstract available

Publication types

Editorial
Introductory Journal Article

MeSH terms

Algorithms
Biocatalysis
Catalytic Domain*
Enzymes / chemistry*
Enzymes / metabolism
Isotopes / chemistry
Kinetics
Models, Chemical*
Models, Molecular*
Substrate Specificity

Substances

Enzymes
Isotopes

Grants and funding