Magnetic Resonance Detection of CD34+ Cells from Umbilical Cord Blood Using a 19F Label

Lucia E Duinhouwer; Bernard J M van Rossum; Sandra T van Tiel; Ramon M van der Werf; Gabriela N Doeswijk; Joost C Haeck; Elwin W J C Rombouts; Mariëtte N D Ter Borg; Gyula Kotek; Eric Braakman; Jan J Cornelissen; Monique R Bernsen

doi:10.1371/journal.pone.0138572

Magnetic Resonance Detection of CD34+ Cells from Umbilical Cord Blood Using a 19F Label

PLoS One. 2015 Sep 22;10(9):e0138572. doi: 10.1371/journal.pone.0138572. eCollection 2015.

Affiliations

¹ Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
² Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
³ Department of Radiology, Erasmus University Medical Centre, Rotterdam, The Netherlands; Department of Nuclear Medicine, Erasmus University Medical Centre, Rotterdam, The Netherlands.

Abstract

Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34+ cells, with 19F-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34+ cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, CD34 / metabolism*
Cell Tracking / methods*
Cells, Cultured
Colony-Forming Units Assay
Fetal Blood / cytology*
Flow Cytometry
Fluorine Radioisotopes
Hematopoietic Stem Cells / chemistry
Hematopoietic Stem Cells / metabolism*
Humans
Lactic Acid / chemistry
Magnetic Resonance Spectroscopy / methods*
Microscopy, Confocal
Nanoparticles / chemistry
Polyglycolic Acid / chemistry
Polylactic Acid-Polyglycolic Acid Copolymer
Reproducibility of Results
Time Factors

Substances

Antigens, CD34
Fluorine Radioisotopes
Polylactic Acid-Polyglycolic Acid Copolymer
Polyglycolic Acid
Lactic Acid

Grants and funding

This work was supported by a grant from TI Pharma and performed within the framework of the TIPharma Project D5-402: ‘The Prograft Study: optimizing the applicability of stem cell therapy.’ The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.