Abstract
This study determined the performance of BD Max StaphSR and the rate of methicillin-resistant Staphylococcus aureus (MRSA) with an unrecognized staphylococcal cassette chromosome mec (SCCmec) right-extremity junction (MREJ) region among 907 methicillin-resistant S. aureus (MRSA) and 900 methicillin-susceptible S. aureus (MSSA) isolates. The rate of mecA/mecC dropout mutants was also evaluated. Only three MRSA isolates (99.7% sensitivity; 904/907) were classified as MSSA by the BD Max StaphSR assay, due to negative results for MREJ. Eight MSSA isolates (99.1% sensitivity; 892/900) were assigned as MRSA. However, six of these MSSA isolates had the mecA gene confirmed by PCR and sequencing (99.8% sensitivity; 898/900). Overall, 7.1% (64/900) of MSSA isolates showed results compatible with a mecA dropout genotype.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins
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Bacteriological Techniques / methods*
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DNA, Bacterial / genetics
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Humans
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Mass Screening / methods*
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Methicillin-Resistant Staphylococcus aureus / genetics
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Methicillin-Resistant Staphylococcus aureus / isolation & purification*
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Molecular Diagnostic Techniques / methods*
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Penicillin-Binding Proteins / deficiency*
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Sensitivity and Specificity
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Staphylococcal Infections / diagnosis*
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Staphylococcal Infections / microbiology
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United States
Substances
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Bacterial Proteins
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DNA, Bacterial
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Penicillin-Binding Proteins
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mecA protein, Staphylococcus aureus
Grants and funding
This study was funded by BD Diagnostics grant number BDS-MXMSRSA. Rodrigo E. Mendes, Amy A. Watters, Paul R. Rhomberg, David J. Farrell, and Ronald N. Jones are employees of JMI Laboratories, which received compensation fees for manuscript preparation.