Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

Rev Soc Bras Med Trop. 2015 Nov-Dec;48(6):776-9. doi: 10.1590/0037-8682-0076-2015.

Abstract

Introduction: This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil.

Methods: Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing.

Results: PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively.

Conclusions: Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brazil
  • DNA, Kinetoplast / genetics*
  • DNA, Protozoan / genetics*
  • Female
  • Insect Vectors / classification
  • Insect Vectors / parasitology*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Psychodidae / classification
  • Psychodidae / parasitology*
  • Trypanosomatina / classification
  • Trypanosomatina / genetics*
  • Trypanosomatina / isolation & purification

Substances

  • DNA, Kinetoplast
  • DNA, Protozoan