Two-tube multiplex real-time reverse transcription PCR to detect six human coronaviruses

Peihua Niu; Jun Shen; Na Zhu; Roujian Lu; Wenjie Tan

doi:10.1007/s12250-015-3653-9

Two-tube multiplex real-time reverse transcription PCR to detect six human coronaviruses

Virol Sin. 2016 Feb;31(1):85-8. doi: 10.1007/s12250-015-3653-9.

Authors

Peihua Niu¹, Jun Shen², Na Zhu¹, Roujian Lu¹, Wenjie Tan³

Affiliations

¹ Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
² Children's Hospital of Fudan University, Shanghai, 200032, China.
³ Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. tanwj28@163.com.

Abstract

Electronic Supplementary Material: Supplementary material is available for this article at 10.1007/s12250-015-3653-9 and is accessible for authorized users.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Animals
Child
Child, Preschool
Coronavirus / genetics*
Coronavirus / isolation & purification*
Coronavirus Infections / diagnosis
Coronavirus Infections / virology*
Haplorhini / virology
Humans
Infant
Infant, Newborn
Middle East Respiratory Syndrome Coronavirus / genetics
Middle East Respiratory Syndrome Coronavirus / isolation & purification
Multiplex Polymerase Chain Reaction / methods*
Real-Time Polymerase Chain Reaction / methods*
Reverse Transcription
Sensitivity and Specificity
Severe Acute Respiratory Syndrome / genetics
Severe Acute Respiratory Syndrome / virology