The RNA Helicase eIF4A Is Required for Sapovirus Translation

J Virol. 2016 Apr 29;90(10):5200-5204. doi: 10.1128/JVI.03174-15. Print 2016 May 15.

Abstract

The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5' untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5' UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions
  • Animals
  • Eukaryotic Initiation Factor-4A / genetics
  • Eukaryotic Initiation Factor-4A / metabolism*
  • Genome, Viral*
  • Mutation
  • Protein Binding
  • RNA, Viral / metabolism
  • Rabbits
  • Reticulocytes / metabolism
  • Sapovirus / genetics*
  • Sapovirus / physiology
  • Sterols / pharmacology
  • Swine
  • Viral Proteins / biosynthesis*
  • Viral Proteins / genetics
  • Virus Replication

Substances

  • 5' Untranslated Regions
  • RNA, Viral
  • Sterols
  • Viral Proteins
  • hippuristanol
  • Eukaryotic Initiation Factor-4A