Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy

F Grimont; D Chevrier; P A Grimont; M Lefevre; J L Guesdon

doi:10.1016/0923-2508(89)90065-x

Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy

Res Microbiol. 1989 Sep;140(7):447-54. doi: 10.1016/0923-2508(89)90065-x.

Authors

F Grimont¹, D Chevrier, P A Grimont, M Lefevre, J L Guesdon

Affiliation

¹ Unité 199 INSERM, Institut Pasteur, Paris.

PMID: 2696056
DOI: 10.1016/0923-2508(89)90065-x

Abstract

The use of acetylaminofluorene-labelled 16 + 23S rRNA (from Escherichia coli) is described for determining rRNA-gene-restriction patterns. The labelled probe allowed molecular fingerprinting of bacteria belonging to diverse phylogenetic branches (Enterobacteriaceae, Haemophilus, Pseudomonas, Acinetobacter, Brucella, Leptospira, Cytophaga, Campylobacter, Methylophaga). The labelled probe can be stored frozen (-20 degrees C) for at least a year and can endure vacuum dessication, ethanol precipitation or lyophilization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

2-Acetylaminofluorene*
Bacteria / classification*
Bacteria / genetics
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Nucleotide Mapping
RNA Probes*
RNA, Ribosomal*
Restriction Mapping

Substances

RNA Probes
RNA, Ribosomal
2-Acetylaminofluorene