Comparison of Xpert® HPV and Hybrid Capture® 2 DNA Test™ for detection of high-risk HPV infection in cervical atypical squamous cells of undetermined significance

J Infect Public Health. 2017 Mar-Apr;10(2):219-223. doi: 10.1016/j.jiph.2016.04.017. Epub 2016 Jun 21.

Abstract

This study compares Xpert® HPV and Hybrid Capture® 2 High-Risk HPV DNA Test™ (hc2) for the detection of high-risk HPV infection in cervical smears. Papanicolaou smears with atypical squamous cells of undetermined significance (ASC-US) constituted the study specimens. Of the 168 ASC-US samples, 134 (79.8%) were from Saudi patients. The hc2 test was positive in 33 (19.6%) of the total patients, 20% among Saudi patients, and 17.6% among non-Saudi patients. Xpert® HPV produced positive results in 30 (17.8%) of the samples. The overall concordance rate between the two tests was 98.2%, and the positive concordance rate was 91%. There were three samples tested positive by hc2 that tested negative by Xpert® HPV. HPV 16, HPV 18/45 and HPV other types were the most common types. Both tests have a reasonable positive concordance rate, although hc2 detected more cases than Xpert® HPV. Xpert® HPV provides a viable alternative to the hc2 test with similar detection results for samples with ASC-US.

Keywords: ASC-US; Atypical squamous cells of undetermined significance; HPV; Human Papilloma virus.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Atypical Squamous Cells of the Cervix / virology*
  • Female
  • Humans
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Papillomaviridae / classification
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / virology
  • Vaginal Smears
  • Young Adult