Microstructural and microglial changes after repetitive mild traumatic brain injury in mice

J Neurosci Res. 2017 Apr;95(4):1025-1035. doi: 10.1002/jnr.23848. Epub 2016 Jul 25.

Abstract

Traumatic brain injury (TBI) is a major public health issue, with recently increased awareness of the potential long-term sequelae of repetitive injury. Although TBI is common, objective diagnostic tools with sound neurobiological predictors of outcome are lacking. Indeed, such tools could help to identify those at risk for more severe outcomes after repetitive injury and improve understanding of biological underpinnings to provide important mechanistic insights. We tested the hypothesis that acute and subacute pathological injury, including the microgliosis that results from repeated mild closed head injury (rmCHI), is reflected in susceptibility-weighted magnetic resonance imaging and diffusion-tensor imaging microstructural abnormalities. Using a combination of high-resolution magnetic resonance imaging, stereology, and quantitative PCR, we studied the pathophysiology of male mice that sustained seven consecutive mild traumatic brain injuries over 9 days in acute (24 hr) and subacute (1 week) time periods. rmCHI induced focal cortical microhemorrhages and impaired axial diffusivity at 1 week postinjury. These microstructural abnormalities were associated with a significant increase in microglia. Notably, microgliosis was accompanied by a change in inflammatory microenvironment defined by robust spatiotemporal alterations in tumor necrosis factor-α receptor mRNA. Together these data contribute novel insight into the fundamental biological processes associated with repeated mild brain injury concomitant with subacute imaging abnormalities in a clinically relevant animal model of repeated mild TBI. These findings suggest new diagnostic techniques that can be used as biomarkers to guide the use of future protective or reparative interventions. © 2016 Wiley Periodicals, Inc.

Keywords: DTI; RRID:AB_2144905; RRID:AB_2314667; RRID:AB_323909; SWI; axial diffusion; inflammation; microhemorrhage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / diagnostic imaging
  • Brain / metabolism
  • Brain / pathology*
  • Brain Injuries, Traumatic / complications
  • Brain Injuries, Traumatic / diagnostic imaging
  • Brain Injuries, Traumatic / pathology*
  • Calcium-Binding Proteins / metabolism
  • Cytokines / genetics
  • Cytokines / metabolism
  • Diffusion Tensor Imaging
  • Disease Models, Animal
  • Gene Expression Regulation / physiology
  • Intracranial Hemorrhages / etiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microfilament Proteins / metabolism
  • Microglia / metabolism
  • Microglia / pathology*
  • Nerve Fibers, Myelinated / pathology*
  • Nitric Oxide Synthase Type II / genetics
  • Nitric Oxide Synthase Type II / metabolism
  • RNA, Messenger / metabolism
  • Statistics, Nonparametric

Substances

  • Aif1 protein, mouse
  • Calcium-Binding Proteins
  • Cytokines
  • Microfilament Proteins
  • RNA, Messenger
  • Nitric Oxide Synthase Type II