HSFY and ZNF280BY show copy number variations within 17 water buffalo populations

X Zhang; H Han; T Zhang; T Sun; Y Xi; N Chen; Y Huang; R Dang; X Lan; H Chen; C Lei

doi:10.1111/age.12514

HSFY and ZNF280BY show copy number variations within 17 water buffalo populations

Anim Genet. 2017 Apr;48(2):221-224. doi: 10.1111/age.12514. Epub 2016 Oct 14.

Authors

X Zhang¹, H Han¹, T Zhang², T Sun¹, Y Xi¹, N Chen¹, Y Huang¹, R Dang¹, X Lan¹, H Chen¹, C Lei¹

Affiliations

¹ College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
² Shaanxi University of Technology, Hanzhong, Shaanxi, 723001, China.

PMID: 27739082
DOI: 10.1111/age.12514

Abstract

Recent transcriptomic analysis of the bovine Y chromosome revealed abundant presence of multi-copy protein coding gene families on the male-specific region of the Y chromosome (MSY). Copy number variations (CNVs) of several MSY genes are closely related to semen quality and male reproduction in cattle. However, the CNVs of MSY genes in water buffalo are largely unknown. Therefore, this study aimed to investigate the CNVs of HSFY and ZNF280BY of 298 buffaloes from 17 populations distributed in China, Vietnam and Laos using quantitative PCR. Our results revealed that the median copy numbers of the HSFY and ZNF280BY genes were 47 (ranging from 20 to 145) and 269 (ranging from 73 to 974) respectively. In conclusion, this study indicated that HSFY and ZNF280BY showed abundant CNVs within swamp buffalo populations.

Keywords: Y-linked genes; buffalo; copy number variations; multi-copy genes.

MeSH terms

Animals
Buffaloes / blood
Buffaloes / classification
Buffaloes / genetics*
China
DNA Copy Number Variations*
Female
Male
Population
Trans-Activators / genetics*
Transcription Factors / genetics*
Vietnam
Y Chromosome*

Substances

Trans-Activators
Transcription Factors