Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs

Adv Exp Med Biol. 2016:924:97-100. doi: 10.1007/978-3-319-42044-8_18.

Abstract

MicroRNAs (miRNAs) in urine are examined as potential biomarkers. We examined the urine samples from 70 individuals (45 males, 25 females, mean age 65 years, range 20-84 years). Of the urine donors, 15 were healthy volunteers, 5 were patients with non-cancer diseases, 50 were patients with different stages of bladder cancer. To examine the spectrum of miRNAs in the cell-free fraction of urine, TaqMan Human miRNA Array Card A v.2.1 was used. A set of 30 miRNAs were found that are constantly present in urine supernatants independently of sex, age and health status of the subjects. We compared this set with miRNAs found in plasma, expressed in kidney and genito-urinary tract. Our results indicate that some miRNA could be transferred from the circulation into urine.

Keywords: Genito-urinary tract; MicroRNA; Plasma; Transrenal passage of microRNAs; Urine supernatant.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / genetics*
  • Biomarkers, Tumor / urine
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Kidney / metabolism*
  • Male
  • MicroRNAs / blood
  • MicroRNAs / genetics*
  • MicroRNAs / urine
  • Middle Aged
  • Reverse Transcriptase Polymerase Chain Reaction
  • Urinary Bladder Neoplasms / diagnosis
  • Urinary Bladder Neoplasms / genetics*
  • Young Adult

Substances

  • Biomarkers, Tumor
  • MicroRNAs