Multiplexed Single-Cell Imaging: Past, Present, and Future

Assay Drug Dev Technol. 2017 Jan;15(1):8-10. doi: 10.1089/adt.2016.765. Epub 2016 Dec 22.

Abstract

Jia-Ren Lin from the Laboratory of Systems Pharmacology at Harvard Medical School was awarded best poster at the annual Society of Biomolecular Imaging and Informatics meeting held in Boston, September 2016. His work focuses on single-cell imaging, especially on developing new methods for simultaneously detecting many antigens, named cyclic immunofluorescence (CycIF). This method could be applied in different stages of drug development, from discovery phase, preclinical research to clinical research. The current works and future directions of CycIF method are summarized in the following overview.

Keywords: cyclic immunofluorescence; drug discovery; multiplexed imaging.

Publication types

  • Review

MeSH terms

  • Animals
  • Cells, Cultured
  • Fluorescent Antibody Technique / methods*
  • Fluorescent Antibody Technique / trends
  • Forecasting
  • Humans
  • Image Cytometry / methods*
  • Image Cytometry / trends