Helicobacter Pylori Promote B7-H1 Expression by Suppressing miR-152 and miR-200b in Gastric Cancer Cells

Gengchen Xie; Wei Li; Ruidong Li; Ke Wu; Ende Zhao; Yu Zhang; Peng Zhang; Liang Shi; Di Wang; Yuping Yin; Rui Deng; Kaixiong Tao

doi:10.1371/journal.pone.0168822

Helicobacter Pylori Promote B7-H1 Expression by Suppressing miR-152 and miR-200b in Gastric Cancer Cells

PLoS One. 2017 Jan 5;12(1):e0168822. doi: 10.1371/journal.pone.0168822. eCollection 2017.

Authors

Gengchen Xie¹, Wei Li¹, Ruidong Li¹, Ke Wu¹, Ende Zhao¹, Yu Zhang², Peng Zhang¹, Liang Shi³, Di Wang¹, Yuping Yin¹, Rui Deng¹, Kaixiong Tao¹

Affiliations

¹ Department of Gastrointestinal Surgery of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.
² Department of Radiology of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.
³ Department of Clinical Laboratory of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.

Abstract

The most common cause of gastric cancer is infection with helicobacter pylori (HP), but the associated molecular mechanism is not well understood. In the present study, we found a marked increase in the expression of B7-H1, a member of the B7 co-stimulatory family of molecules that bind to programmed death-1 (PD-1) and play a critical immunoregulatory role in the cell-mediated immune response, in HP-positive gastric cancer tissue. Infection of cultured gastric cancer cells with HP promoted B7-H1 expression and inhibited miR-152 and miR-200b expression. We further demonstrated that these two miRNAs targeted B7-H1 mRNA and suppressed B7-H1 expression in gastric cancer cells. Finally, B7-H1 expression was found to correlate with miR-152 and miR-200b levels in gastric tumor tissues from human patients. Our findings suggest a novel mechanism by which HP infection promotes gastric cancer and also suggest potential targets, i.e., miR-152 and miR-200b, for the prevention and treatment of gastric cancer.

MeSH terms

3' Untranslated Regions / genetics
B7-H1 Antigen / genetics
B7-H1 Antigen / metabolism*
Cell Line, Tumor
Flow Cytometry
Fluorescent Antibody Technique
Helicobacter pylori / pathogenicity*
Humans
MicroRNAs / genetics
MicroRNAs / metabolism*
Real-Time Polymerase Chain Reaction
Stomach Neoplasms / genetics
Stomach Neoplasms / metabolism*
Stomach Neoplasms / microbiology*

Substances

3' Untranslated Regions
B7-H1 Antigen
MIRN152 microRNA, human
MIRN200 microRNA, human
MicroRNAs

Grants and funding

This work was supported by National Natural Science Foundation of China (http://www.nsfc.gov.cn/publish/portal1/): No. 81172294, KT, Role in conceptualization, investigation, resources, writing review & editing, visualization, supervision, project administration and funding acquisition; No. 81572413, KT, Role in conceptualization, investigation, resources, writing review & editing, visualization, supervision, project administration and funding acquisition; No. 81402357, EZ, Role in methodology and software.