Immuno-PCR technology for detection of natural human antibodies against Lec disaccharide

Artem V Maerle; Daria V Voronina; Kira L Dobrochaeva; Oxana E Galanina; Leonid P Alekseev; Nicolai V Bovin; Sergei K Zavriev; Dmitriy Yu Ryazantsev

doi:10.1007/s10719-016-9751-6

Immuno-PCR technology for detection of natural human antibodies against Le^c disaccharide

Glycoconj J. 2017 Apr;34(2):199-205. doi: 10.1007/s10719-016-9751-6. Epub 2017 Jan 14.

Authors

Artem V Maerle¹, Daria V Voronina², Kira L Dobrochaeva², Oxana E Galanina², Leonid P Alekseev³, Nicolai V Bovin², Sergei K Zavriev², Dmitriy Yu Ryazantsev²

Affiliations

¹ JSC NPF DNA-Technology, Moscow, Russia. maerle@yandex.ru.
² Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
³ NRC Institute of Immunology, Federal Biomedical Agency of Russia, Moscow, Russia.

PMID: 28091939
DOI: 10.1007/s10719-016-9751-6

Abstract

The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described. The sensitivity of the assay for determination of low-affinity anti-Le^C IgM has been found to be 4 ng/ml (~100 pg per sample), thus being two orders of magnitude higher compared to the conventional ELISA with the same antigen.

Keywords: Glycans; Immuno-PCR; LeC; Natural antibodies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Disaccharides / chemistry*
Disaccharides / immunology
Humans
Immunoglobulin M / analysis*
Immunoglobulin M / immunology
Polymerase Chain Reaction / methods*

Substances

Disaccharides
Immunoglobulin M