Glutathione is a highly efficient thermostabilizer of poliovirus Sabin strains

Vaccine. 2017 Mar 7;35(10):1370-1372. doi: 10.1016/j.vaccine.2017.01.070. Epub 2017 Feb 8.

Abstract

Glutathione (GSH) is the most abundant thiol peptide in animal cells and has a critical role in antioxidation. GSH was reported to be essential for stabilization of some enteroviruses, including poliovirus (PV), during viral morphogenesis. Here, we explored the potential use of GSH as a thermostabilizer of oral poliomyelitis vaccine (OPV) formulations. GSH significantly protected the three types of PV from heat-inactivation in a concentration-dependent manner. At a GSH concentration of 20mM, nearly complete protection was observed against heating temperatures up to 53°C for 2min.GSH also markedly protected PV1 from heat-inactivation and this up to 6 h at temperatures of 44°C and 46°C and 3 h at 48°C. The fact that GSH is naturally present at high concentration in the human body makes it an efficient candidate stabilizer for OPV formulations.

Keywords: Cold chain; Glutathione; Poliomyelitis; Sabin; Vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Drug Stability*
  • Excipients / metabolism*
  • Glutathione / metabolism*
  • Microbial Viability / radiation effects
  • Poliovirus / physiology
  • Poliovirus / radiation effects
  • Poliovirus Vaccine, Oral / chemistry*
  • Poliovirus Vaccine, Oral / immunology*
  • Temperature*
  • Virus Inactivation

Substances

  • Excipients
  • Poliovirus Vaccine, Oral
  • Glutathione