Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

F Abali; M Stevens; A G J Tibbe; L W M M Terstappen; P N van der Velde; R B M Schasfoort

doi:10.1016/j.ab.2017.05.021

Isolation of single cells for protein therapeutics using microwell selection and Surface Plasmon Resonance imaging

Anal Biochem. 2017 Aug 15:531:45-47. doi: 10.1016/j.ab.2017.05.021. Epub 2017 May 22.

Authors

F Abali¹, M Stevens², A G J Tibbe², L W M M Terstappen³, P N van der Velde⁴, R B M Schasfoort⁵

Affiliations

¹ Medical Cell Biophysics Group, MIRA Institute, Faculty of Science and Technology, University of Twente, PO Box 217, 7500AE Enschede, The Netherlands.
² VyCAP, Abraham Rademakerstraat 41, 7425PG Deventer, The Netherlands.
³ Medical Cell Biophysics Group, MIRA Institute, Faculty of Science and Technology, University of Twente, PO Box 217, 7500AE Enschede, The Netherlands. Electronic address: l.w.m.m.terstappen@utwente.nl.
⁴ IBIS Technologies, Pantheon 5, 7521 PR Enschede, The Netherlands.
⁵ Medical Cell Biophysics Group, MIRA Institute, Faculty of Science and Technology, University of Twente, PO Box 217, 7500AE Enschede, The Netherlands; Interfluidics BV, Duizendblad 28, 7483 AL Haaksbergen, The Netherlands.

PMID: 28545866
DOI: 10.1016/j.ab.2017.05.021

Abstract

Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.

Keywords: Antibody/protein excretion; EpCAM; Hybridoma; McSPRInter; Microwell; SPR cytometry; SPRi; Single cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Separation* / instrumentation
Cell Separation* / methods
Humans
Surface Plasmon Resonance* / instrumentation
Surface Plasmon Resonance* / methods
Tissue Array Analysis* / instrumentation
Tissue Array Analysis* / methods