Short-pulse neodymium:yttrium-aluminium garnet (Nd:YAG 1064nm) laser irradiation photobiomodulates mitochondria activity and cellular multiplication of Paramecium primaurelia (Protozoa)

Eur J Protistol. 2017 Oct;61(Pt A):294-304. doi: 10.1016/j.ejop.2017.06.003. Epub 2017 Jun 19.

Abstract

Few studies exist to explore the potential photobiomodulation (PBM) effect of neodymium:yttrium-aluminium garnet (Nd:YAG) laser irradiation using a flat-top handpiece delivery system. In this study, we explored the photobiomodulation effect of that laser, on Paramecium primaurelia. The parameters for the different study groups were: 0.50W, 10Hz, 100msp, 30J/cm2; 0.75W, 10Hz, 100msp, 45J/cm2; 1.00W, 10Hz, 100msp, 60J/cm2; 1.25W, 10Hz, 100msp, 75J/cm2 and 1.50W, 10Hz, 100msp, 90J/cm2. Our results suggest that only the parameter 0.5W, 10Hz, 100msp, 30J/cm2 positively photobiomodulates the Paramecium cells inducing an increment in oxygen consumption, endogenous ATP synthesis and fission rate rhythm. Applying the laser energy with parameters of 1.25W, 10Hz, 100msp, 75J/cm2 and 1.50W, 10Hz, 100msp, 90J/cm2, induce adverse effect on the Paramecium cells, which protect themselves through the increase in Heat Shock Protein-70 (HSP70). The data presented in our work support our assumption that, when using appropriate parameters of irradiation, the 1064nm Nd:YAG laser with flat-top handpiece could be a valuable aid for effective clinical application of PBM.

Keywords: ATP; Heat Shock Protein; Low Level Laser Therapy; Low Light Laser Therapy; Photobiomodulation; Phototherapy.

MeSH terms

  • Aluminum / chemistry
  • Cell Proliferation / radiation effects
  • Lasers, Solid-State*
  • Mitochondria / metabolism
  • Neodymium / chemistry
  • Oxygen Consumption / radiation effects
  • Paramecium / cytology
  • Paramecium / metabolism
  • Paramecium / radiation effects*
  • Yttrium / chemistry

Substances

  • Neodymium
  • Yttrium
  • Aluminum