Mapping of DNA markers close to the fragile site on the human X chromosome at Xq27.3

M Patterson; S Kenwrick; S Thibodeau; K Faulk; M G Mattei; J F Mattei; K E Davies

doi:10.1093/nar/15.6.2639

Mapping of DNA markers close to the fragile site on the human X chromosome at Xq27.3

Nucleic Acids Res. 1987 Mar 25;15(6):2639-51. doi: 10.1093/nar/15.6.2639.

Authors

M Patterson, S Kenwrick, S Thibodeau, K Faulk, M G Mattei, J F Mattei, K E Davies

Abstract

We report the identification of a new RFLP detected by the DNA probe MN12, which is linked to both the fragile site on the X chromosome at Xq27.3 and the highly polymorphic locus detected by St14 (DXS52). In situ mapping confirms the localisation of MN12 distal to the fragile site. A detailed physical analysis of this region of the X chromosome using pulsed-field gel electrophoresis has shown that MN12, St14 and DX13 (DXS15) are physically linked within a region of 470kb. A long range restriction map around the MN12 locus reveals at least two candidate HTF islands, suggesting the existence of expressed sequences in this region.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Chromosome Fragile Sites
Chromosome Fragility*
Female
Humans
Male
Nucleic Acid Hybridization
Nucleotide Mapping
Pedigree
Plasmids
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length*
X Chromosome*