Adiponectin prevents orthodontic tooth movement in rats

Sigrid Haugen; Kristin Matre Aasarød; Astrid Kamilla Stunes; Mats Peder Mosti; Tanya Franzen; Vaska Vandevska-Radunovic; Unni Syversen; Janne Elin Reseland

doi:10.1016/j.archoralbio.2017.08.009

Adiponectin prevents orthodontic tooth movement in rats

Arch Oral Biol. 2017 Nov:83:304-311. doi: 10.1016/j.archoralbio.2017.08.009. Epub 2017 Aug 26.

Authors

Sigrid Haugen¹, Kristin Matre Aasarød², Astrid Kamilla Stunes³, Mats Peder Mosti⁴, Tanya Franzen⁵, Vaska Vandevska-Radunovic⁶, Unni Syversen⁷, Janne Elin Reseland⁸

Affiliations

¹ Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Oslo, Norway. Electronic address: sigrid.haugen@odont.uio.no.
² Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway; Clinic of Medicine, St. Olav's Hospital, Trondheim, Norway. Electronic address: kristin.aasarod@ntnu.no.
³ Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway; Clinic of Medicine, St. Olav's Hospital, Trondheim, Norway. Electronic address: kamilla.stunes@ntnu.no.
⁴ Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway; Clinic of Medicine, St. Olav's Hospital, Trondheim, Norway. Electronic address: mats.p.mosti@ntnu.no.
⁵ Department of Orthodontics, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway. Electronic address: tanyaf@odont.uio.no.
⁶ Department of Orthodontics, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway. Electronic address: vaskav@odont.uio.no.
⁷ Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway; Department of Endocrinology, St. Olav's Hospital, Trondheim, Norway. Electronic address: unni.syversen@ntnu.no.
⁸ Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Oslo, Norway. Electronic address: j.e.reseland@odont.uio.no.

PMID: 28866437
DOI: 10.1016/j.archoralbio.2017.08.009

Abstract

Objective: The objective was to examine the effects of repetitive local administration of adiponectin on experimental tooth movement in rats.

Materials and methods: The maxillary right first molar of male Wistar rats (n=24) was moved mesially for 14days, with local adiponectin injections (0.2 or 2μg) every third day. Micro-computed tomography was performed at days 0, 6 and 14 and molar movement, bone density and bone volume fraction were calculated from the scans. Changes in extracellular matrix collagen and cell numbers in the periodontal ligament were analyzed histologically, and levels of circulating cytokines were measured by Luminex and ELISA.

Results: Adiponectin injections induced a reduction in tooth movement after 12 and 14days compared to controls. No tooth movement was observed between days 3 and 14 in the group receiving the highest dosage (2μg) of adiponectin. Differences in bone density and bone volume fractions between treatment and control groups were not identified. Relative size and morphology of collagen fibrils, and cell number in the periodontal region after adiponectin injections were unchanged compared to controls. Levels of circulating adiponectin or other selected factors in plasma were not influenced by the adiponectin injections.

Conclusions: Submucosal injections of adiponectin prevented experimental tooth movement in rats. The effect was dosage-dependent and local. Adiponectin injections caused no detectable changes in bone density, periodontal cell number or collagen content.

Keywords: Adiponectin; Experimental tooth movement.

MeSH terms

Adiponectin / administration & dosage
Adiponectin / pharmacology*
Animals
Bone Density
Cytokines / metabolism
Enzyme-Linked Immunosorbent Assay
Male
Periodontal Ligament / cytology
Rats
Rats, Wistar
Tooth Movement Techniques*
X-Ray Microtomography

Substances

Adiponectin
Cytokines