Abstract
We report effects of adding insulin-like growth factor I (IGF-I) and methionyl human growth hormone (GH), alone or in combination, to adult bovine articular chondrocytes plated at high density. Purified human and synthetic IGF-I stimulated chondrocyte DNA and proteoglycan synthesis. GH had no effect on either process. However, GH added in combination with IGF-I increased proteoglycan, cell-associated proteoglycan, and keratan sulfate synthesis over levels observed with IGF-I alone. IGF-I and GH did not alter the hydrodynamic size of proteoglycans or synthesis of collagen. Our results show that GH and IGF-I act together to stimulate adult chondrocyte extracellular matrix synthesis.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Cartilage, Articular / cytology*
-
Cartilage, Articular / metabolism
-
Cartilage, Articular / physiology
-
Cattle
-
Cell Division / drug effects
-
DNA / biosynthesis
-
Glycosaminoglycans / biosynthesis
-
Growth Hormone / analogs & derivatives*
-
Growth Hormone / pharmacology
-
Hormones / pharmacology*
-
Human Growth Hormone
-
Humans
-
Insulin-Like Growth Factor I / pharmacology*
-
Keratan Sulfate / biosynthesis
-
Proline / metabolism
-
Proteoglycans / biosynthesis
-
Somatomedins / pharmacology*
-
Thymidine / metabolism
Substances
-
Glycosaminoglycans
-
Hormones
-
Proteoglycans
-
Somatomedins
-
Human Growth Hormone
-
Insulin-Like Growth Factor I
-
Growth Hormone
-
DNA
-
Keratan Sulfate
-
Proline
-
Thymidine