Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells

J Nat Med. 2018 Jan;72(1):280-289. doi: 10.1007/s11418-017-1161-y. Epub 2017 Dec 5.

Abstract

A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from D-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a "co-culture" in which the genus Nocardia was cultured in the presence of an animal cell line.

Keywords: Actinomycetes; Amide; Co-culture; Nocardia.

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Biosynthetic Pathways
  • Cell Line
  • Coculture Techniques
  • Host-Pathogen Interactions
  • Macrophages / microbiology
  • Mice
  • Nocardia / genetics
  • Nocardia / metabolism*
  • Nocardia Infections / metabolism
  • Nocardia Infections / microbiology
  • Pantothenic Acid / analogs & derivatives*
  • Pantothenic Acid / biosynthesis
  • Pantothenic Acid / chemistry
  • Pantothenic Acid / isolation & purification*
  • Phylogeny

Substances

  • Bacterial Proteins
  • Pantothenic Acid