Objective: To investigate the effect of exposure to particulate matter ≤10 μm in aerodynamic diameter (PM(10)) on sperm quality in different stages of sperm development. Methods: This cross-sectional study included 1 827 patients attending the reproductive medicine center in Renmin Hospital of Wuhan University during April 2013 to January 2015. Air pollution data from January 2013 to January 2015 was obtained from the database of Wuhan Municipal Environmental Protection Bureau. The generalized linear model was employed to assess the association between each exposure variables and sperm parameters for several exposure windows (0-9, 10-14, 15-69, 70-90, 0-90 days before sampling) . Results: The average levels of PM(10) was (116.2±71.6) μg/m(3) during the research period. Sperm volume was (75.4±49.1) ×10(6)/ml in sample population, (29.4±16.2) % in progressive motility and (51.8±21.6) % in total motility. Exposure to PM(10) was inversely associated with sperm concentration (β:-0.319; 95%CI:-0.529,-0.046) during 70-90 lag days. PM(10) exposure during the 0-90 lag days was significantly associated with progressive motility (β:-0.312; 95%CI:-0.527,-0.097) and total motility (β:-0.347; 95%CI:-0.636,-0.059) after adjusted for age, education level, BMI, smoking, abstinence time, temperature, humidity and season. Conclusion: Exposure to PM(10) was associated with statistically significant decrements in sperm concentration and motility, and the adverse impact on sperm concentration was significantly in early phases of spermatogenesis.
目的： 探索PM(10)暴露在精子生成的不同阶段对精液质量的影响。 方法： 以2013年4月至2015年1月赴武汉大学人民医院生殖医学中心就诊的1 827名武汉籍男性作为研究对象。收集武汉市污染物的浓度和气象数据，控制年龄、BMI等混杂因素后，建立广义线性模型，研究PM(10)暴露与取精前（lag）0~9、10~14、15~69、70~90 d以及0~90 d对精液质量的影响。 结果： 2013年1月至2015年1月，PM(10)的日平均浓度为（116.2±71.6）μg/m(3)。研究对象的精液浓度为（75.4±49.1）×10(6)个/ml，前向运动的精子比例为（29.4±16.2）%，精子总活力为（51.8±21.6）%。PM(10)暴露在lag70~90 d与精液浓度呈负相关（β:-0.319; 95%CI:-0.529，-0.046），在lag0~90 d的暴露区间，PM(10)暴露与前向运动精子比例（β:-0.312; 95%CI:-0.527，-0.097）以及精子的总活力（β:-0.347; 95%CI:-0.636，-0.059）亦呈负相关。 结论： 武汉市大气PM(10)暴露可能与精液浓度和精子活力下降有关，且对于精子浓度的影响在精子发生的前期更为显著。.
Keywords: Cross-sectional study; PM(10); Particulate matter; Sperm count; Spermatogenesis.