The lectin pathway and coagulation in lung cancer patients undergoing lobectomy - A randomised controlled trial

Julie Brogaard Larsen; Anne Troldborg; Thomas Decker Christensen; Christine Lodberg Hvas; Steffen Thiel; Anne-Mette Hvas

doi:10.1016/j.thromres.2018.01.042

The lectin pathway and coagulation in lung cancer patients undergoing lobectomy - A randomised controlled trial

Thromb Res. 2018 Mar:163:92-99. doi: 10.1016/j.thromres.2018.01.042. Epub 2018 Feb 6.

Authors

Julie Brogaard Larsen¹, Anne Troldborg², Thomas Decker Christensen³, Christine Lodberg Hvas⁴, Steffen Thiel², Anne-Mette Hvas⁵

Affiliations

¹ Centre for Haemophilia and Thrombosis, Department of Clinical Biochemistry, Aarhus University Hospital, Denmark.
² Department of Biomedicine, Aarhus University, Denmark.
³ Department of Cardiothoracic and Vascular Surgery, Aarhus University Hospital, Denmark; Department of Clinical Medicine, Aarhus University, Denmark.
⁴ Department of Anaesthesiology and Intensive Care Medicine, Randers Regional Hospital, Denmark.
⁵ Centre for Haemophilia and Thrombosis, Department of Clinical Biochemistry, Aarhus University Hospital, Denmark; Department of Clinical Medicine, Aarhus University, Denmark. Electronic address: am.hvas@dadlnet.dk.

PMID: 29407633
DOI: 10.1016/j.thromres.2018.01.042

Abstract

Background: Lectin pathway proteases activate coagulation and may theoretically play a role in the increased thrombosis risk in cancer, which is especially high during surgery.

Aims: To investigate lectin pathway proteins during lung cancer surgery, the influence of low molecular weight heparin (LMWH) on lectin pathway proteins, and correlations between lectin pathway proteins and coagulation.

Methods: Fifty lung cancer patients undergoing video-assisted thoracoscopic surgery lobectomy were randomised to LMWH, n = 26, or no anticoagulant (control), n = 24. Pre-, intra- and postoperative lectin pathway protein concentrations (mannose-binding lectin (MBL), H- and M-ficolin, collectin liver-1, MBL-associated serine protease (MASP)-1, -2 and -3, MBL-associated proteins MAp44 and MAp19) were assessed using a time-resolved immunofluorometric assay, and fibrinogen, fibrin d-dimer, and thrombin generation were analysed.

Results: For all proteins except MASP-1, concentrations decreased during surgery in both groups; most markedly M-ficolin which decreased 49% (median: 2836 [quartiles:2297-3505] to 1424 [1187-2199] ng/ml) (LMWH group) and 43% (2974 [2539-3510] to 1685 [1391-2076] ng/ml) (control group), while MBL decreased 12% (1936 [823-2801] to 1702 [676-2830] ng/ml) (LMWH) and 23% (1526 [250-2412] to 1175 [229-1947]) (controls). No differences in postoperative change were observed between groups except for MAp19 (p = 0.03) which decreased 9% (401 [337-467] to 364 [288-416] ng/ml) (LMWH) vs 28% (370 [272-468] to 268 [212-379] ng/ml) (controls). No correlation was found between lectin pathway proteins and coagulation (r = -0.23-0.28, p > 0.06) except for M-ficolin and fibrinogen (r = 0.29-0.36, p = 0.01-0.04).

Conclusion: Lectin pathway proteins were influenced by surgery but not by LMWH. No consistent correlation was found between lectin pathway proteins and coagulation.

Keywords: Blood coagulation; Complement pathway, mannose-binding lectin; Complement system protein; Heparin, low molecular weight heparin; Neoplasms.

Publication types

Randomized Controlled Trial

MeSH terms

Aged
Aged, 80 and over
Anticoagulants / pharmacology
Anticoagulants / therapeutic use*
Blood Coagulation / physiology*
Female
Humans
Lectins / metabolism*
Lung Neoplasms / genetics*
Lung Neoplasms / surgery
Male
Middle Aged

Substances

Anticoagulants
Lectins