Analysis of quetiapine in human plasma using fluorescence spectroscopy

Islam M Mostafa; Mahmoud A Omar; Dalia M Nagy; Sayed M Derayea

doi:10.1016/j.saa.2018.02.019

Analysis of quetiapine in human plasma using fluorescence spectroscopy

Spectrochim Acta A Mol Biomol Spectrosc. 2018 May 5:196:196-201. doi: 10.1016/j.saa.2018.02.019. Epub 2018 Feb 6.

Authors

Islam M Mostafa¹, Mahmoud A Omar², Dalia M Nagy², Sayed M Derayea²

Affiliations

¹ Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt. Electronic address: dr-islam@mu.edu.eg.
² Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.

PMID: 29448172
DOI: 10.1016/j.saa.2018.02.019

Abstract

A simple and sensitive spectrofluorimetric method has been development for the assurance of quetiapine fumarate (QTF). The proposed method was utilized for measuring the fluorescence intensity of the yellow fluorescent product at 510nm (λ_ex 470nm). The fluorescent product has resulted from the nucleophilic substitution reaction of QTF with 4-chloro-7-nitrobenzofurazane (NBD-Cl) in Mcllvaine buffer (pH7.0). The diverse variables influencing the development of the reaction product were deliberately changed and optimized. The linear concentration range of the proposed method was of 0.2-2.0μgml^-1.The limits of detection and quantitation were 0.05 and 0.17μgml^-1, respectively. The proposed method was applied for the assurance of QTF in its tablets without interference from basic excipients. In addition, the proposed method was used for in vitro analysis of the QTF in spiked human plasma, the percent mean recovery was (n=3) 98.82±1.484%.

Keywords: NBD-Cl; Quetiapine fumarate; Spectrofluorimetric; Spiked plasma.

MeSH terms

4-Chloro-7-nitrobenzofurazan
Acetone
Humans
Hydrogen-Ion Concentration
Limit of Detection
Linear Models
Quetiapine Fumarate / blood*
Reproducibility of Results
Spectrometry, Fluorescence / methods*

Substances

Acetone
Quetiapine Fumarate
4-Chloro-7-nitrobenzofurazan