Transcriptome analysis of silver, palladium, and selenium stresses in Pantoea sp. IMH

Abstract

Heavy metal contamination is a significant environmental issue. Using bacteria for removal and reduction of heavy metals is an attractive alternative owing to its low-cost and eco-friendly properties. However, the mechanisms of resistance to and reduction of Ag(I), Pd(II), and Se(IV), especially in the same strain, remain unclear. Here, Pantoea sp. IMH was examed for its reduction of Ag(I), Pd(II), and Se(IV) to nanoparticles (NPs), and the molecular mechanism was investigated by transcriptome analysis. The results revealed that genes encoding binding, transport, catalytic activity, and metabolism were differentially expressed in cells exposed to Ag(I), Pd(II), and Se(IV). The same resistance mechanisms for all metals included multiple stress resistance protein BhsA and glutathione detoxification metabolism. However, zinc transport protein and sulfate metabolism played an important role in the resistance to cationic metals (Ag⁺ and Pd²⁺), while the oxalate transporter and arsenic resistance mechanisms were specifically involved in the resistance to and reduction of anion (SeO₃^2-). In addition, Ag(I) was speculated to be reduced to AgNPs by glucose and cytochrome CpxP was involved in Pd(II) reduction. Our results provided new clues on the mechanisms of resistance to and reduction of Ag(I), Pd(II), and Se(IV).

Keywords: Bioreduction; Metal resistance; Nanoparticles; Palladium; Selenium; Silver.