An electro-conductive plane heating element for rapid thermal lysis of bacterial cells

J Microbiol Methods. 2018 Oct:153:99-103. doi: 10.1016/j.mimet.2018.09.012. Epub 2018 Sep 20.

Abstract

In the process of developing a point-of-care testing device, we fabricated a plane heating element using carbon paste capable of thermal lysis. The plane heating element can generate heat of 150 °C within 60 s at 0.4 A power supply within 8 W. The developed plane heating element was very effective in the extraction of DNA from both Gram-negative and Gram-positive bacteria which was further confirmed by agarose gel electrophoresis. In this approach, we aimed to eliminate expensive, time-consuming and labor-intensive procedures for the lysis of bacterial cells to extract nucleic acids to detect pathogenic bacteria accurately and swiftly.

Keywords: Carbon paste; POCT; Plane heating element; Sample preparation; Thermal lysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / cytology*
  • Carbon
  • DNA, Bacterial / isolation & purification*
  • Gram-Negative Bacteria / cytology
  • Gram-Positive Bacteria / cytology
  • Hot Temperature*
  • Nucleic Acid Amplification Techniques
  • Point-of-Care Systems
  • Specimen Handling / instrumentation*
  • Specimen Handling / methods*

Substances

  • DNA, Bacterial
  • Carbon