In the process of developing a point-of-care testing device, we fabricated a plane heating element using carbon paste capable of thermal lysis. The plane heating element can generate heat of 150 °C within 60 s at 0.4 A power supply within 8 W. The developed plane heating element was very effective in the extraction of DNA from both Gram-negative and Gram-positive bacteria which was further confirmed by agarose gel electrophoresis. In this approach, we aimed to eliminate expensive, time-consuming and labor-intensive procedures for the lysis of bacterial cells to extract nucleic acids to detect pathogenic bacteria accurately and swiftly.
Keywords: Carbon paste; POCT; Plane heating element; Sample preparation; Thermal lysis.
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