Ultrasensitive ELISA for the detection of hCG based on assembled gold nanoparticles induced by functional polyamidoamine dendrimers

Weiguo Wang; Junmin Li; Changzhi Dong; Yafang Li; Qiuye Kou; Jinwu Yan; Lei Zhang

doi:10.1016/j.aca.2018.08.038

Ultrasensitive ELISA for the detection of hCG based on assembled gold nanoparticles induced by functional polyamidoamine dendrimers

Anal Chim Acta. 2018 Dec 26:1042:116-124. doi: 10.1016/j.aca.2018.08.038. Epub 2018 Aug 23.

Authors

Weiguo Wang¹, Junmin Li¹, Changzhi Dong², Yafang Li³, Qiuye Kou³, Jinwu Yan⁴, Lei Zhang⁵

Affiliations

¹ School of School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, PR China.
² Joint International Research Laboratory of Synthetic Biology and Medicine, South China University of Technology, Guangzhou, 510006, PR China; Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 Rue J-A de Baïf, 75205, Paris Cedex 13, France.
³ The Sixth Affiliated Hospital of Sun Yat-Sen University, 26th Yuancun Road II, Guangzhou, 510655, PR China.
⁴ School of School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, PR China; Joint International Research Laboratory of Synthetic Biology and Medicine, South China University of Technology, Guangzhou, 510006, PR China. Electronic address: yjw@scut.edu.cn.
⁵ School of School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, PR China; Joint International Research Laboratory of Synthetic Biology and Medicine, South China University of Technology, Guangzhou, 510006, PR China. Electronic address: lzhangce@scut.edu.cn.

PMID: 30428978
DOI: 10.1016/j.aca.2018.08.038

Abstract

Amplifying the signal of ELISA is important in the early disease diagnosis. Herein we report an ultrasensitive ELISA applying for the detection of hCG based on the assemble of AuNPs induced by functional PAMAM. The AuNP-PAMAM probe shows a competitive advantage sensitivity of 0.03 IU L^-1 compared to traditional ELISA and mAb₁-AuNP-HRP probe. The line range is ranged from 0.1 to 6.4 IU L^-1. Moreover, the precision and reproducibility and specificity of AuNP-PAMAM probe are also eligible for the detection of hCG. The assembled AuNPs was firstly used in the signal enhancement in immunoassay.

Keywords: Assemble; Enzyme-linked immunosorbent assay; Gold nanoparticles; Polyamidoamine dendrimers; Signal amplification.

MeSH terms

Chorionic Gonadotropin / analysis*
Dendrimers / chemistry*
Enzyme-Linked Immunosorbent Assay*
Gold / chemistry*
Humans
Metal Nanoparticles / chemistry*
Polyamines / chemistry*

Substances

Chorionic Gonadotropin
Dendrimers
Poly(amidoamine)
Polyamines
Gold