Comparison of analytical performances of the Roche Cobas 6800 CT/NG assay with the Abbott m2000 Real Time CT/NG assay for detecting Chlamydia trachomatis and Neisseria gonorrhoeae

J Med Microbiol. 2019 Feb;68(2):197-200. doi: 10.1099/jmm.0.000909. Epub 2019 Jan 3.

Abstract

The Roche Cobas 6800 CT/NG assay was compared to the Abbott m2000 Real Time CT/NG assay for detecting Chlamydia trachomatis and Neisseria gonorrhoeae in 714 specimens referred to the bacteriology laboratory at Geneva University Hospitals, between November 2017 and March 2018, and in nine external quality controls for molecular diagnostics (seven from QCMD Glasgow and two from UK NEQAS). For C. trachomatis, the sensitivity of C6800 compared to m2000 was 100 % (95 % confidence interval [CI], 97.5 to 100 %), the specificity was 99.1 % (95 % CI, 98.0 to 99.7 %). For N. gonorrhoeae, the sensitivity of the C6800 compared to m2000 was 100 % (95 % CI, 90.5 to 100 %), whereas the specificity was 99.7 % (95 % CI, 98.9 to 99.9 %). The C6800 CT/NG assay appears to perform with great accuracy the detection of C. trachomatis and N. gonorrhoeae.

Keywords: Abbott m2000; Chlamydia trachomatis; Cobas 6800; Neisseria gonorrhoeae.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Anal Canal / microbiology
  • Child
  • Chlamydia trachomatis / genetics
  • Chlamydia trachomatis / isolation & purification*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Neisseria gonorrhoeae / genetics
  • Neisseria gonorrhoeae / isolation & purification*
  • Oropharynx / microbiology
  • Predictive Value of Tests
  • Prospective Studies
  • Real-Time Polymerase Chain Reaction / standards*
  • Rectum / microbiology
  • Retrospective Studies
  • Sensitivity and Specificity
  • Urine / microbiology
  • Vagina / microbiology
  • Young Adult