Abstract
Metabolic activation by several forms of purified cytochrome P-450 of aflatoxin B1 to a product(s) mutagenic to Salmonella typhimurium TA100 was examined. Of the 5 forms of cytochrome P-450 purified from liver microsomes of untreated and PCB-treated male rats, a constitutive form purified from untreated male rats, P-450-male, and a high-spin form of cytochrome P-450, P-448-H, from PCB-treated rats were highly active.
MeSH terms
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Aflatoxin B1
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Aflatoxins / metabolism*
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Aflatoxins / pharmacology
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Animals
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Biotransformation
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Carcinogens / metabolism*
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Cytochrome P-450 Enzyme System / isolation & purification
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Cytochrome P-450 Enzyme System / metabolism*
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Male
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Microsomes, Liver / drug effects
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Microsomes, Liver / metabolism*
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Mutagenicity Tests
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Mutagens*
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Mutation*
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NADPH-Ferrihemoprotein Reductase / isolation & purification
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NADPH-Ferrihemoprotein Reductase / metabolism
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Polychlorinated Biphenyls / pharmacology
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Rats
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Rats, Inbred Strains
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Salmonella typhimurium / drug effects
Substances
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Aflatoxins
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Carcinogens
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Mutagens
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KC 400
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Cytochrome P-450 Enzyme System
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Aflatoxin B1
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Polychlorinated Biphenyls
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NADPH-Ferrihemoprotein Reductase