Increased CD8+CD27+perforin+ T cells and decreased CD8+CD70+ T cells may be immune biomarkers for aplastic anemia severity

Suwen Zhao; Yuping Zhang; Guixuan Huang; Weifeng Luo; Yan Li; Yankai Xiao; Ming Zhou; Yumiao Li; Jing Lai; Yangqiu Li; Bo Li

doi:10.1016/j.bcmd.2019.03.009

Increased CD8⁺CD27⁺perforin⁺ T cells and decreased CD8⁺CD70⁺ T cells may be immune biomarkers for aplastic anemia severity

Blood Cells Mol Dis. 2019 Jul:77:34-42. doi: 10.1016/j.bcmd.2019.03.009. Epub 2019 Mar 30.

Authors

Suwen Zhao¹, Yuping Zhang², Guixuan Huang³, Weifeng Luo⁴, Yan Li⁵, Yankai Xiao¹, Ming Zhou², Yumiao Li², Jing Lai⁶, Yangqiu Li⁷, Bo Li⁸

Affiliations

¹ Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China.
² Department of Hematology, Guangzhou First Municipal People's Hospital, The Second Affiliated Hospital of South China University of Technology, Guangzhou, China.
³ Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China.
⁴ Guangzhou Blood Center, Guangzhou, China.
⁵ Department of Cardiology, First Affiliated Hospital, Jinan University, Guangzhou, China.
⁶ Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, China.
⁷ Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China; Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China; Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, China. Electronic address: yangqiuli@hotmail.com.
⁸ Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China; Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China. Electronic address: libo517@jnu.edu.cn.

PMID: 30953940
DOI: 10.1016/j.bcmd.2019.03.009

Abstract

Objectives: Aplastic anemia (AA) is T cell immune-mediated autoimmune disease. Aberrant T cell activation involves an imbalance in T cell homeostasis in AA. However, whether the T cell activation molecule CD27 and its ligand CD70 participate in the immune pathogenesis of AA remains ill defined.

Methods: The frequencies of CD27/CD70 and perforin/granzyme B in different T cell subsets were detected in AA patients and healthy individuals by flow cytometry.

Results: We first time demonstrate a significantly elevated proportion of CD27⁺ and significantly decreased CD70⁺ T cells from AA. Changed frequency of CD27⁺ and CD70⁺ in different T cell subsets appeared to be associated with AA severity. In very severe aplastic anemia (VSAA) and severe aplastic anemia (SAA), increased CD8⁺CD27⁺ T cells present with a cytotoxic effector phenotype by elevating perforin proportion.

Conclusions: Elevated proportion of CD27 in T cells may contribute to distinct immune pathogenesis for different severities of AA. The CD8⁺CD27⁺perforin⁺ T cells combined with CD8⁺CD70⁺ T cells may serve as an immune biomarker for AA severity estimation.

Keywords: Aplastic anemia; CD27; CD70; Perforin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Anemia, Aplastic / diagnosis*
Anemia, Aplastic / immunology
Anemia, Aplastic / metabolism*
Biomarkers
CD27 Ligand / metabolism*
CD4-Positive T-Lymphocytes / immunology
CD4-Positive T-Lymphocytes / metabolism
CD8-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / metabolism*
Female
Flow Cytometry
Humans
Lymphocyte Activation
Lymphocyte Count
Male
Middle Aged
Perforin / metabolism*
Severity of Illness Index
T-Lymphocyte Subsets / immunology
T-Lymphocyte Subsets / metabolism*
Tumor Necrosis Factor Receptor Superfamily, Member 7 / metabolism*
Young Adult

Substances

Biomarkers
CD27 Ligand
CD70 protein, human
Tumor Necrosis Factor Receptor Superfamily, Member 7
Perforin