Inactivation of oral biofilms using visible light and water-filtered infrared A radiation and indocyanine green

Thomas Burchard; Lamprini Karygianni; Elmar Hellwig; Marie Follo; Thomas Wrbas; Annette Wittmer; Kirstin Vach; Ali Al-Ahmad

doi:10.4155/fmc-2018-0522

Inactivation of oral biofilms using visible light and water-filtered infrared A radiation and indocyanine green

Future Med Chem. 2019 Jul;11(14):1721-1739. doi: 10.4155/fmc-2018-0522. Epub 2019 Aug 1.

Authors

Thomas Burchard¹, Lamprini Karygianni², Elmar Hellwig³, Marie Follo⁴, Thomas Wrbas³, Annette Wittmer⁵, Kirstin Vach⁶, Ali Al-Ahmad³

Affiliations

¹ Department of Prosthetic Dentistry, Center for Dental Medicine, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
² Clinic of Preventive Dentistry, Periodontology & Cariology, Center of Dental Medicine, University of Zurich, Switzerland.
³ Department of Operative Dentistry & Periodontology, Center for Dental Medicine, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
⁴ Department of Hematology, Oncology & Stem Cell Transplantation, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
⁵ Department of Hygiene & Microbiology, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
⁶ Department of Medical Biometry & Statistics, Faculty of Medicine, University of Freiburg, Germany.

PMID: 31368351
DOI: 10.4155/fmc-2018-0522

Abstract

Aim: To investigate the antimicrobial photodynamic therapy (aPDT) of visible light and water-filtered infrared A radiation in combination with indocyanine green (ICG) on planktonic oral microorganisms as well as on oral biofilm. Methods: The irradiation was conducted for 5 min in combination with ICG. Treatment with chlorhexidine served as a positive control. The number of colony forming units and bacterial vitality were quantified. Results: All tested bacterial strains and salivary bacteria were killed at a level of 3log_10. The colony forming units of the initial mature oral biofilms were strongly reduced. The high bactericidal effect of aPDT was confirmed by live/dead staining. Conclusion: The aPDT using visible light and water-filtered infrared A radiation and ICG has the potential to treat periodontitis and peri-implantitis.

Keywords: ICG; VIS+wIRA; aPDT; antimicrobial photodynamic therapy; indocyanine green; oral biofilm; visible light and water-filtered infrared A.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Bacteria / drug effects*
Biofilms / drug effects
Humans
Indocyanine Green / chemistry
Indocyanine Green / pharmacology*
Light*
Microbial Sensitivity Tests
Photochemotherapy
Photosensitizing Agents / chemistry
Photosensitizing Agents / pharmacology*
Water / chemistry*

Substances

Anti-Bacterial Agents
Photosensitizing Agents
Water
Indocyanine Green