Objective: To investigate the clinical significance of bone marrow microvessel density(MVD) and angiogenesis related factors in multipic myeloma(MM).
Methods: Twenty cases of MM and 20 cases of simple fracture were selected and enrolled in MM group and control group respectively. The clinical data and results of laboratorial tests were collected; the bone marrow MVD of patients was detected by using the modified plastic-embedded pathologic sections of bone marrow tissue and histochemistry staining, the expression levels of amgiogenesis-related factors including VEGF, TNF-α, HGF, TGF-α, TGF-β1, bFGF, Ang-Ⅰ, Ang-Ⅱ in bone marrow supernatant were detected by ELISA; the mRNA expression levels of above-mentioned cytokines in bone marrow mononuclear cells were detected by real time-PCR; the pearson correlation analysis was used to analyze the correlation of MVD with VEGF, HGF and bFGF levels.
Results: The MVD in MM group was significantly higher than that in control group (P＜0.001); the mRNA expression of VEGF, TGF-α, TGF-β1 and HGF in bone marrow mononuclear cells of MM group was higher than that of control group(P＜0.001); the levels of VEGF, HGF, bFGF and THF-α in bone marrow supernatant of MM group were higher than those in control group(P＜0.05), moreover, the MVD positively correlated with levels of VEGF, HGF and bFGF in bone marrow(r=0.488, 0.472 and 0.457).
Conclusion: The MVD and levels vessel-related factors in bone marrow supernatant of MM patients increase, among which the levels of VEGF and HGF in bone marrow supernatant are consistant with those mRNA expression level in bone marrow mononuclear cells, moreover, the MVD possitively cerrelates with levels of VEGF, HGF and bFGF in bone marrow supernatant, suggesting that the changes of bone marrow microenvironment vassel-related factors play an important role in angiogenesis and pathogenesis of multiple myeloma.
题目: 骨髓微血管密度及血管相关生成因子在多发性骨髓瘤中的意义.
目的: 探讨骨髓微血管密度及血管相关生成因子在多发性骨髓瘤中的临床意义.
方法: 选取2014年1月到2017年12月就诊于本院就诊的多发性骨髓瘤（MM）患者和单纯骨折患者各20例并分别纳入MM组和对照组，收集患者入院基本临床资料，通过改良的塑料包埋骨髓活组织病理切片和免疫组织化学染色检测患者骨髓微血管密度（MVD）；ELISA法检测骨髓上清VEGF、TNF-α、HGF、TGF-α、TGF-β1、bFGF、ANG-Ⅰ、ANG-Ⅱ等血管生成相关因子的水平；real-time-PCR检测骨髓单个核细胞的上述因子mRNA表达水平，pearson相关分析MVD与骨髓上清VEGF、HGF、bFGF水平的相关性.
结果: MM组MVD明显高于对照组（P＜0.001），MM组骨髓单个核细胞VEGF、TGF-α、TGF-β1、HGF mRNA表达均明显高于对照组（P＜0.001），MM组骨髓上清VEGF、HGF、bFGF和TNF-α水平均高于对照组（P＜0.05），并且MVD与骨髓VEGF、HGF、bFGF水平呈正相关（r=0.488，0.472和0.457）.
结论: MM患者MVD和骨髓上清中血管相关因子水平升高，其中VEGF、HGF水平和骨髓单个核细胞mRNA表达水平一致，并且MVD和骨髓上清VEGF、HGF、bFGF水平呈正相关，提示骨髓微环境血管生成因子的变化在多发性骨髓瘤血管生成和疾病发生中起重要的作用.