Expression of serum lncRNA-Xist in patients with polycystic ovary syndrome and its relationship with pregnancy outcome

Abstract

Objective: Long non-coding RNA X-inactive specific transcript (LncRNA Xist) plays a pivotal role in various types of human diseases, while its involvement in polycystic ovary syndrome (PCOS) remains unknown. In the study we aimed to investigate if downregulate Xist expression is involved in PCOS and is correlated with adverse pregnant outcomes in PCOS.

Materials and methods: In this study, expression of lncRNA Xist in peripheral blood collected from both PCOS patients and controls were detected by qRT-PCR. Diagnostic values of Xist expression for PCOS were evaluated by ROC curve analysis. Correlation between Xist expression and pregnant outcomes of PCOS patients was analyzed.

Results: Compared with controls, Xist was significantly downregulated in patients with PCOS but not in patients with other types of diseases. Xist expression can be used to effectively distinguish PCOS patients from controls. Reduced expression level of Xist was significantly correlated with adverse pregnant outcomes of PCOS patients but not healthy controls.

Conclusion: The downregulation of Xist expression may be involved in PCOS and is correlated with adverse pregnant outcomes in PCOS.

Keywords: Diagnostic tests routine; Endocrine system diseases; Long noncoding RNA; Polycystic ovary syndrome; Pregnancy outcome.