Evaluation of a high-speed but low-throughput RT-qPCR system for detection of SARS-CoV-2

J Sakai; N Tarumoto; Y Orihara; R Kawamura; M Kodana; N Matsuzaki; R Matsumura; K Ogane; T Kawamura; S Takeuchi; K Imai; T Murakami; S Maesaki; T Maeda

doi:10.1016/j.jhin.2020.05.025

Evaluation of a high-speed but low-throughput RT-qPCR system for detection of SARS-CoV-2

J Hosp Infect. 2020 Aug;105(4):615-618. doi: 10.1016/j.jhin.2020.05.025. Epub 2020 May 22.

Authors

J Sakai¹, N Tarumoto², Y Orihara³, R Kawamura³, M Kodana³, N Matsuzaki³, R Matsumura⁴, K Ogane³, T Kawamura³, S Takeuchi³, K Imai¹, T Murakami⁵, S Maesaki¹, T Maeda⁶

Affiliations

¹ Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan; Centre of Clinical Infectious Disease and Research, Saitama Medical University, Saitama, Japan.
² Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan; Centre of Clinical Infectious Disease and Research, Saitama Medical University, Saitama, Japan. Electronic address: tarumoto@saitama-med.ac.jp.
³ Department of Clinical Laboratory, Saitama Medical University Hospital, Saitama, Japan.
⁴ Centre of Clinical Infectious Disease and Research, Saitama Medical University, Saitama, Japan.
⁵ Department of Microbiology, Saitama Medical University, Saitama, Japan.
⁶ Centre of Clinical Infectious Disease and Research, Saitama Medical University, Saitama, Japan; Department of Clinical Laboratory, Saitama Medical University Hospital, Saitama, Japan; Department of Laboratory Medicine, Saitama Medical University, Saitama, Japan.

Abstract

With the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), a high-speed and convenient detection technology should be at the forefront of medical care worldwide. This study evaluated the usefulness of GeneSoC, a compact, high-speed reciprocal flow quantitative reverse transcription polymerase chain reaction system, for the detection of SARS-CoV-2. The results support the use of this system for the rapid identification of SARS-CoV-2. This approach can contribute to the strategic selection of initial management strategies for patients with COVID-19.

Keywords: COVID-19; GeneSoC; RT-qPCR; SARS-CoV-2.

MeSH terms

Betacoronavirus / genetics*
Betacoronavirus / isolation & purification*
COVID-19
Clinical Laboratory Techniques / methods*
Coronavirus Infections / diagnosis*
Coronavirus Infections / genetics*
Humans
Japan
Pandemics
Pneumonia, Viral / diagnosis*
Pneumonia, Viral / genetics*
Real-Time Polymerase Chain Reaction / methods*
SARS-CoV-2
Sensitivity and Specificity