Regulation of Actg1 and Gsta2 is possible mechanism by which capsaicin alleviates apoptosis in cell model of 6-OHDA-induced Parkinson's disease

Biosci Rep. 2020 Jun 26;40(6):BSR20191796. doi: 10.1042/BSR20191796.

Abstract

The present study aimed to identify the gene expression changes conferred by capsaicin in the cell model of 6-OHDA-induced Parkinson's disease, to disclose the molecular mechanism of action of capsaicin. We used capsaicin-treated and paraffin-embedded wax blocks containing substantia nigra tissue from 6-OHDA-induced Parkinson's disease rats to analyze transcriptional changes using Affymetrix GeneChip Whole Transcript Expression Arrays. A total of 108 genes were differentially expressed in response to capsaicin treatment, and seven of these genes were selected for further analysis: Olr724, COX1, Gsta2, Rab5a, Potef, Actg1, and Acadsb, of which Actg1 (actin gamma 1) was down-regulated and Gsta2 (Glutathione S-transferase alpha 2) was up-regulated. We successfully overexpressed Actg1 and Gsta2 in vitro. CCK-8 detection and flow cytometry demonstrated that overexpression of Actg1 and Gsta2 increased apoptosis in the 6-OHDA-induced Parkinson's disease cell model. The imbalance between Actg1 and Gsta2 may be one of the mechanisms of cell damage in Parkinson's disease (PD). Capsaicin can protect the cells and reduce the apoptosis rate by regulating Actg1 and Gsta2.

Keywords: Actg1; Gsta2; Parkinson's disease; apoptosis; neuroprotection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / genetics
  • Actins / metabolism*
  • Animals
  • Antiparkinson Agents / pharmacology*
  • Apoptosis / drug effects*
  • Capsaicin / pharmacology*
  • Cell Line, Tumor
  • Dihydroxyphenylalanine / analogs & derivatives*
  • Dihydroxyphenylalanine / toxicity
  • Disease Models, Animal
  • Gene Expression Regulation
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism*
  • Humans
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Neurons / drug effects*
  • Neurons / enzymology
  • Neurons / pathology
  • Parkinson Disease / drug therapy*
  • Parkinson Disease / enzymology
  • Parkinson Disease / genetics
  • Rats
  • Signal Transduction
  • Substantia Nigra / drug effects
  • Substantia Nigra / enzymology
  • Substantia Nigra / pathology

Substances

  • ACTG1 protein, human
  • Actins
  • Antiparkinson Agents
  • Isoenzymes
  • 6-hydroxydopa
  • Dihydroxyphenylalanine
  • Glutathione Transferase
  • glutathione S-transferase alpha
  • Capsaicin